ISOLATION OF GERANYL FLAVONOID AND ANTIOXIDANT ACTIVITIES OF BREADFRUIT LEAF EXTRACT (ARTOCARPUS ALTILIS)

Abstract

   Breadfruit is widely cultivated and used as a traditional medical for treating variousdiseases. From the ethyl acetate extract of breadfruit leaf, three geranyl flavonoidwere isolated comprising 2-geranyl-3,4,2',4-tetrahydroxydihydrochalcone AA1, 1-(2,4-dihydroxyphenyl)-3-[8-hydroxy-2-methyl-2-(4-methyl-3-pentenyl)-2H-1-benzopyran-5-yl]-1-propanone AA2 and (S)-Sophoraflavanone A AA3. Thebiosynthesized pathway of these isolated compounds were proposed through the keystep condensation between geranyl pyrophosphate and flavonoid moiety. The in vitroantioxidant activities revealed both methanol and ethyl acetate extract possessedantioxidant effect and the ethyl acetate extract was the most effective with IC50 value79.3 and 9.32 g/mL in comparison to positive control, Vitamin C with IC50 value4.39 and Trolox 11.80 g/mL in both DPPH and ABTS methods, respectively.

Keywords: Artocarpus altilis, breadfruit, geranyl flavonoid, DPPH, ABTS.