DETERMINATION OF VITAMIN D2 IN MUSHROOMS BY HIGHPERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) AND ENRICHMENT OF VITAMIN D2 IN MUSHROOMS

Abstract

   An analytical procedure based on the use of high-performance liquid chromatographywas applied for the determination of vitamin D2 in mushrooms. The detection limitwas 0,027ppm and quantification limit was 0,083ppm. The recovery of vitamin D2 was87%. The method is applicable for establishing data for some mushrooms. WhiteBeechMushrooms (Hypsizygus tessellatus), king trumpet mushroom (Pleurotuseryngii), Enokitake (Flammulina velutipes) were irradiated with pulsed ultraviolet UVlight. Irradiation of each side of the mushroomsfor 6h, was found to be the optimumperiod of irradiation in this conversion.The highest vitamin D2 content (43,5g/100gmushroom) was observed in king trumpet mushroom irradiated with pulsed ultravioletUV lightat 6h.

Keywords: mushrooms, vitamin D2, ergocalciferol, pulsed ultraviolet UV light, foodanalysis.