DETERMINATION OF VITAMIN A CONTENTS OF PROCESSED CEREALS IN HUE CITY AND NEARLY AREAS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

      In this paper, we report the result of research and development analytical methods of vitamin A in processed cereals by high performance liquid chromatography (HPLC) with fluorescence detecter (RF). The research results show tat the determination is measured at 348 nm/370 nm wavelength, reversed phase C18 column (150 mm x 4,6 mm, 5 μm), at room temperature, mobile phase: MeOH: H2O (90:10 by volume), 20L sample injection volume, flow rate 0.8 mL/min. The highly sensitive method good repeatability (RSD% < 2%, n = 8), low detection limit, LOD = 0.003 ppm. Between the concentration of vitamin A and peak area with good linear correlation in the range 0.05 ÷ 10.00 ppm with R2 = 0.9998. Results of quality control methods on real samples showed that the method has a good right to withdraw from the 96 ÷ 101%.