RAPID AND SIMULTANEOUS DETERMINATION OF CEFADROXIL, CEFALEXIN, CEFACLOR IN TABLETS BY NEAR AND MIDLE INFRARED SPECTROSCOPY

Abstract

     In this study, the simultaneous quantitative analysis of cefadroxil, cephalexin, cefaclor in tablets using multivarite linear regression based on near infrared spectral has been developed. The same model of partial least squares (PLS) with 6 separate vector matrix is built with standard matrix of samples (22 samples containing 4 analytes together with total of excipients) were applied for individual and/or simultaneous determination of cefadroxil, cephalexin, cefaclor in samples. A matrix of test samples including 9 samples created by known amount of components was used to assess the accuracy of the PLS model. The results indicated the relative error varied from 1.1 to 15%. The optimum experimental conditions were spectral region: 3600 - 2800 cm-1; volume ratio of sample/ KBr: 98 (w/w); 14mg of pellet form. The analytical results of 3 commercial drug samples containing 1 of 3 actives by means of near-infrared spectral and chromatographic methods LC-MS mass spectrometry showed the small relative error (<15%) between two methods. The showed results can be used for rapid and simultaneous determination of the active ingredients of cefadroxil, cephalexin, cefaclor in the pharmaceutical tablets.

Từ khoá: phương pháp hồng ngoại gần, hồi qui đa biến tuyến tính, bình phương tối thiểu từng phần, LC/MS- orbitrap