ANALYSIS OF BETA-BLOCKERS IN URINE SAMPLE USING LC/MS/MS TECHNIQUE

Abstract

    An analytical method has been developed for analysis of ten beta-blockers in urine sample. Extraction of ten beta-blockers was carried out based on the dispersive solid phase extraction sample preparation method. Determination was performed using liquid chromatography electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS). Mother ion and two fragment ion transitions of each beta-blocker were identified by direct injection to MS mode and by multiple reactions monitoring (MRM) mode, respectively. Spiking experiments was carried out to determine the recovery, precision and limit of quantitation (LOQ) of the method. The overall recoveries of all beta-blockers were between 62% and 102% with relative standard deviation lower than 15%. The limit of quantitation (LOQ) for all studied compounds was acceptable in comparison with regulations of World Anti Doping Agency.