MANUFACTURING TEST KITS COUPLED WITH HAND HELD SPECTROMETER FOR ONSITE QUANTITATIVE ANALYSIS OF NITRITE AND NITRATE IN WATER SAMPLES

Abstract

    Monitoring of Nitrogen species is very important in assessment of eutrophication ofwater environment. Beside the online monitoring of environment's chemicalparameters by automated monitoring station, rapid determination of nitrogencompounds in fields can prevent the transformation of species during preservation ofsamples. This study focuses on the manufacturing of test kits couples with hand heldspectrometer for rapid determination of nitrite and nitrate in water samples. The testkits for nitrite based on spectrometric standard methods using power modified Griessreagent and simple equipment. Nitrate was analyzed the same test kit of nitritefollowed the reduction to nitrite by zinc powder. The concentration of nitrate wascalculated from the differences of total obtained nitrite and nitrite determined in thepresence of zinc powder to correct the below 100% of reduction's yield. Thevalidations of test kits were carried out to confirm the LOD and LOQ satisfied withQCVN 08:2008 for nitrite and nitrate levels in surface water. The other57characteristics such as accuracy, ruggedness, robustness and stability have been alsoexamined. The results collected by using test kits and DPM- MT instrument (KyoritsuWater Analysis) proved that the test kits can be successfully applied for trade portablespectrometer for onsite quantitative analysis of nitrite and nitrate in water samples.

Keywords: nitrite, nitrate test kit, portable spectrometer, onsite quantitative analysis,water samples.