STUDY ON THE SIMULTANEOUS DETERMINE CYTIDINE 5’- MONOPHOSPHATE DISODIUM AND URIDINE 5’- MONOPHOSPHATE DISODIUM IN THE LYOPHILIZED INJECTION BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) METHOD

Abstract

A method is described for the determination of cytidine 5’-monophosphate disodium(CMP) and uridine 5’-monophosphate disodium (UMP) in the lyophilized injection by HighPerformance Liquid Chromatography (HPLC). The optimal condition for the determination of(CMP) and (UMP) were found: The mobile phase composition KH2PO4(0,01M): H2O was(95:5); the flow rate of the mobile phase was 1mL/min, the column oven was maintained at260C. Under suitable conditions, the method gained high reproducibility of the CMP andUMP peak current: RSD = 2% (n=6), and low limit of detection ( 3σ)and UMP, and recoveries for the CMP and UMP are in the range of 99,0% to 100,4%. Thedeveloped approach is applied for the determination of the trace amounts of the CMP andUMP in the lyophilized injection in Vietname.