RESEARCH ON IDENTIFYING OF ANAMMOX BACTERIA IN THE BIOMASS OF ANAMMOX ENRICHMENT SYSTEM IN LEATHER AND SHOE RESEARCH INSTITUTE, MINISTRY OF INDUSTRY AND TRADE USING MOLECULAR BIOLOGY TECHNIQUES

Abstract

   Anammox is one of the newly found drivers known to contribute to the biogeochemical nitrogen cycle. In the marine environment, more than 50% of nitrogenous compounds are reportedly converted into nitrogen gas via the anammox pathway. Several methods have been used to detect the presence and activity of anammox bacteria in the environment, including 16S rRNA gene-based approaches or based on their lipids, functional genes, and unique reaction pathways. Here, we report a molecular method using the primer sets that were published for detecting the genes encoding 16S rRNA gene and subunit of the hydrazine oxidoreductase (hzsA) in anammox bacteria.  16S rRNA gene sequences and hzsA gene sequences were retrieved from anammox en-richment cultures system in center of analysis and environmental technology, Leather and Shoe Research Institute, Ha Noi. All sequences were demonstrated that anammox bacteria which were belonged to Candidatus Brocadia genus presented in our system.