STUDY ON DETERMINATION OF β-ECDYSTERONE AND CHEMICAL CONSTITUENTS FROM THE ROOT OF ACHYRANTHES BIDENTATA BLUME, COLLECTED IN HUNGYEN

Abstract

In this study, we have developed a quantitative method β-ecdysterone in root samples of Achyranthes
bidentata Blume colleted in Hungyen. Only a sample (NT03) had β-ecdysterone content of 0.051 ± 0.001 %,
while the others were below 0.03%. Four compounds including acutoside A (1), calendoloside E (2), β
ecdysterone (3) and daucosterol (4) were isolated from NT03 sample. Their chemical structures were
determined by MS, 1D-, 2D-NMR spectral methods, which were compared with the data reported in
previous literature.