Effect of CD3/CD28 coated beads and Interleukin-2 on the proliferation of human Peripheral Blood Mononuclear Cell (PBMC)

Abstract

Immunotherapy is considered a promising approach in cancer treatment. The patient’s immune cells are isolated, expansion, manipulation, and then transfer back to the patient. Several immune cells are ex vivo proliferated, such as Natural Killer cells (NK cells), Chimeric Antigen Receptor T cells (CART cells) and engineered T Cell Receptor cells (TCR cells). The applications of cellular immunotherapy require in vitro culture and proliferation of immune cells. In this study, we evaluated the effect of different concentrations of IL-2 on PBMC proliferation as well as the ability to activate PBMC using CD3/CD28 coated magnetic beads with a ratio of 01 bead to 03 cells. The results showed that there was no significant difference in PBMC proliferation at different concentrations of IL-2 in the first four days of culture. The addition of CD3/CD28 beads and 30U/mL IL-2 into the medium showed the ability to activate and proliferate cells, the number of PBMC doubled after 04 days of activation in which the CD3⁺ cell population increased 03 times, reaching nearly 70% in the PBMC population, the CD4⁺ and CD8⁺ cell populations also increased in number and reached the same proportion, about 30%.