AGROBACTERIUM-MEDICATED TRANSFORMATION OF THE SSIV GENE TO FRIABLE EMBRYOGENIC CALLI FROM KM140 CASSAVA VARIETY WITH THE HELP OF BACTERIA

Abstract

Currently, gene technology would be the best method to improve starch productivity in cassava. Starch synthase (SS) enzymes are coded by five gene groups, named GBSS (granule-bound starch synthase), SSI, SSII, SSIII, and SSVI. In which, each  (SS) enzyme variant has different components and has a certain role in amylopectin synthesis, specifically, SSIV gene would be used to increase the size and content of starch granules. In this study, SSIV gene was isolated from KM140 cassava variety, inserted into pBI121-C54:SSIV:NOST vector and transformed to friable embryogenic callus (FEC) via Agrobacterium tumefacines. Seven transgenic cassava strains which had positive results with PCR testing were formed as the intinial results.