XANTHINE OXIDASE AND NITRIC OXIDE INHIBITIVE EFFECT OF STILBENE ISOLATED FROM GNETUM MONTANUM MARKGR.

Abstract

Gnetum montanum Markgr, a member of the Gnetaceae family, has garnered attention for its
potential therapeutic properties in traditional medicine. This study aimed to evaluate the xanthine
oxidase (XO) and nitric oxide (NO) inhibitory activities of three stilbenes (isorhapotigenin, gnetifolin
E, and (+)-ampelopsin F) isolated from the bark and leaves of G. montanum, a folk medicinal plant
used to treat gout. The structures of the compounds were determined based on NMR and ESI-MS
spectra. Bioactivity results showed that all three compounds have inhibitory activity against XO and
NO, of which gnetifolin E has good activity with IC50 values of 21.42 ± 0.27 and 24.38 ± 1.11µg/mL,
respectively. This is the first report on the xanthine oxidase and nitric oxide inhibitory activity of the
compounds from G. montanum.