Identification of Basidiomycetes laccase genes in Nam Cat Tien forest soil by metagenomics

Abstract

It was reported that there were 0.1 – 1% microorganism discovered by traditional cultivation, 99% others were not known cause of difficuties or impossible for growing in labratory’s conditions. Studying on microorganisms without culture was an aim of modern microbiology diversity. This research was used metagenomics method to access the diversity of laccase genes of mushrooms in Nam Cat Tien forest soil. The technique was simple, fast and cheap such as extracting and purifing DNA directly from soil, cloning by using retrograde primers and analyzing sequences of genes library. We have identified successfully laccase-like gene from samples collected in Nam Cat Tien forest.