TOTAL PHENOLIC CONTENT, AND BIOLOGICAL POTENTIAL OF THE METHANOLIC EXTRACT FROM THE LICHEN PYRENULA SP.
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https://doi.org/10.54607/hcmue.js.23.5.5681(2026)Abstract
Pyrenula lichens are highly diverse and widely distributed in Vietnam; however, their chemical composition and biological activities remain largely unexplored. In this study, preliminary screening of major groups of secondary metabolites, the phenolic content, antioxidant, antibacterial, and a-glucosidase inhibitory activities of Pyrenula lichen collected in Ho Chi Minh City were investigated. The presence of alkaloids, terpenoids, coumarins, quinones, tannins, phenols, and flavonoids in the extract was confirmed by qualitative screening. The total phenolic content (TPC) was determined to be 30.26 ± 0.51 mg GAE/g extract. In terms of bioactivities, the extract exhibited moderate antioxidant activity in the DPPH scavenging assay, with an IC50 value of 97.4 ± 8.5 µg/mL. Besides, the alpha-glucosidase inhibition of the extract (IC50 = 46.5 ± 3.6 µg/mL) suggests its potential for antidiabetic management. Moreover, the extract showed strong antibacterial activity against Staphylococcus aureus, confirmed by an inhibition zone diameter of 20.9 ± 0.1 mm and a MIC value of 15.62 μg/mL. These results show that Pyrenula sp. is a promising source for further investigation of biologically active secondary metabolites and their potential applications.Downloads
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