RESEARCH ON USING IN VITRO TECHNIQUES TO PROPAGATE LADY SLIPPER ORCHID (PAPHIOPEDILUM TRANLIENIANUM)

Abstract

The difference between  lady's slipper orchids and others is easy to realize beause of the special morphology of flowers. There is only one sac-like petal, as a slipper in the flower, therefore this orchids species named as "lady's slipper orchids". Paphiopedilum tranlienianum is one of endemic orichids in Vietnam. The fruits, which were pollinated after 9 months, were selected for experiments. The embryos were cultivated and germinated on MS medium, supplemented with 30 g/l sugar, 1 g/l activated carbon, 5.5 g/l agar, 150 ml/l coconut water. The rapid multiplication phase was supplemented with growth regulator as: BAP 4 mg/l or BAP 4 mg/l, combined with 0.4-0.6 mg/l kinetin. In this media, the hormone had a great influence on the ability to create many buds with the coefficient of germination is 79; Shoot height is 2.46 cm; tree is fat and green. The  root is induced the most in the MS medium which was supplemented with 30 g/l sugar, 5.5 g/l agar, 150 ml/l coconut water and 1 mg/l concentration of NAA or IAA, which can enhance the growth  of root in vitro.