ISOLATION OF Bacillus WITH ACTIVE ACTIVE ANTI-FISHING COLLECTIONS Colletotrichum spp. CAUSES ANTHRACNOSE ON PAPAYA FRUIT POSTHARVEST

Abstract

The study was conducted to isolate and select Bacillus strains with antifungal activity against Colletotrichum spp. causing anthracnose disease on papaya fruit after harvest. As a result, 12 strains of Bacillus were isolated from rhizopheric soil samples of papaya roots grown in Vinh Long province. The study used diffusion well method on agar and double culture method to determine antagonistic activity of isolated Bacillus strains on Colletotrichum spp. The results showed that 6/12 Bacillus strains showed antagonistic activity against Colletotrichum spp. Two bacterial strains BHL21 and BHL23 exhibited the highest antagonistic activity against Colletotrichum spp. with inhibition zone diameters of 20.6 mm and 18.6 mm, respectively. Based on morphological, biochemical characteristics and PCR and sequencing results of 16S rRNA gene, strain BHL21 is 100% homologous to B.  methylotrophicus and strain BHL23 is 99.87% homologous to B. amyloliquefaciens on Genbank.