MORPHOLOGICAL AND SEQUENCE CHARACTERISTIC OF SOME TAXONOMIC GENES OF Paphiopedilum canhii FROM LAOS

Abstract

Paphiopedilum is a genus outstanding of Venus orchid. This is a small genus but has a complex taxonomy with many different views. Species Paphiopedilum canhii was discovered in 2010 and considered as a endemic species to Vietnam. Studies of the species Paphiopedilum canhii reveal their very distinct taxonomic position. To improve classification of this species, a combination of criteria had to be used. Currently, in addition to the traditional method of classification by morphology, the birth of DNA barcode has greatly supported taxonomic studies. This study presents the results of plant morphology analysis of Paphiopedilum canhii samples which were collected in Laos. Simultaneously identify and analyze 5 taxonomic gene sequences matK, rbcL, rpoC1, ITS and trnH-psbA. The results of analysis of gene sequences and taxa showed that ITS and trnH-psbA are suitable indicators when studying on the taxonomy of Paphiopedilum. The branches of the taxonomic tree clearly reflect the relationship of relatives and species. They are grouped in accordance with the sections, subgenus compared with the published taxonomic classification by the morphological of previous studies. Three indicators such as matK, rbcL, rpoC1 for mixed grouping results between sections and subgenus.