TRANSFORMATION AND ASSESSMENT OF Melia azedarach L. LINES CARRYING GA20-OXIDASE GENE PROMOTING BY GmPrP2 PROMOTER

Abstract

Melia azedarach L. is a forest tree with many uses, in Vietnam it is grown quite popularly. In this study, GA20 encoding for the enzyme GA20-oxidase which plays an important role in GA biosynthesis in plant cells was transferred to the plant by vector GmPrP2:GA20ox.. The results of checking the presence of the GA20ox gene by PCR showed that there were 15 lines carrying the gene. Transgene-carrying sinus lines were evaluated for expression at the molecular level by RT-PCR and also showed that the GA20ox gene was active. Assessing the growth of transgenic lines after 8 weeks of age, lines T16, T20 and T22 had an increase in height and number of nodes by 150 - 200% compared to the control. Anatomy of the 8th stem showed that the density of xylem fibers and the number of cells on the xylem fibers of the transgenic lines were higher than that of the control lines, and the cell wall thickness of the lines were similar. These results demonstrate the role of the GmPrP2 promoter with the GA20ox gene on growth and biomass improvement in woody plants.