CHARACTERISTICS OF rrn16S, trnK-UUU GENES AND PHYLOGENY OF Adinandra megaphylla Hu SPECIES COLLECTED IN LAO CAI PROVINCE, VIETNAM

Abstract

Adinandra megaphylla Hu is a plant commonly referred to in Vietnamese as “Sum Lá Lớn” or “Hồng đạm Sapa” which belongs to the Adinandra genus. The extracts from the leaves and stems of this species contain several bioactive compounds which present pharmacological activities. The rrn16S and trnK-UUU genes in the chloroplast genome of A. megaphylla Hu were analyzed to determine the genetic relationship among the species collected in Vietnam and Adinandra species published on GenBank. The results show that the rrn16S and trnK-UUU genes in the chloroplast genome of A. megaphylla Hu are 1490 and 2591 nucleotides, respectively. The nucleotide sequences of the rrn16S and trnK-UUU of A. megaphylla Hu in Northern Vietnam were determined to have high similarity with several species of the Adinandra genus, and this plant is most closely related to A. bockiana. A combination of the rrn16S and trnK-UUU genes can be a DNA barcoding candidate for determining the genetic relationship of A. megaphylla Hu. These results are fundamental for further studies on A. megaphylla Hu and other species of the Adinandra genus and contribute to research on the diversity and conservation of genetic resources in Vietnam.