INTERACTION AND DATA MINING OF EXPRESSION OF THE NUCLEAR FACTOR-Y SUBUNITS IN CHICKPEA (Cicer arietinum)

Abstract

Protein - protein interaction and data mining of the expression patterns of genes were considered as the major reliable tools to construct the theory of the gene functions. Here, the interactions between Nuclear factor - Y (NF-Y) subunits and the expression profiles of corresponding genes found in chickpea (Cicer arietinum) were performed. Based on the interactome database, we predicted that members of CaNF-YB subunits can slightly interact with members of CaNF-YC subunit to construct the heterodimer, which then merges with CaNF-YA subunit to create the CaNF-Y complex as fully reported in other plant species. Among them, four formulas of protein-protein interaction, including [(CaNF-YB19/-YC01)/-YA06], [(CaNF-YB19/-YC01)/-YA02], [(CaNF-YB18/-YC01)/-YA06] and [(CaNF-YB18/-YC01)/-YA02] were predicted. Next, the conserved domain of interacted CaNF-YA subunit consisted of two distinct sub-domains, including the DNA binding sub-domain and the CaNF-YB/YC interaction domain. The conserved domains of interacted CaNF-YB and -YC subunits were reported to harbor two regions, CaNF-YA interaction sub-domain and CaNF-YC or -YB interaction sub-domain. Of our interest, data mining of the previous transcriptome atlas revealed that genes encoding interacted CaNF-Y subunits were highly expressed in at least one organ. Taken together, out study would provide the important information for the further characterization of genes encoding CaNF-Y subunits.