IN-VITRO STUDY ON THE ANTIOXIDANT CAPACITY OF ALKALINE IONIZED WATER

Tóm tắt

This study assessed the antioxidant capacity of alkaline ionized water produced at laboratory scale using a Kangen TRIM ION H-2 device and a re-filtered domestic water source from Can Tho city. Two approaches were used. First, ORP measurements showed the alkaline ionized water had a negative ORP, which theory links with strong reducing and anti-oxidation potential. Yet this effect did not last when the water stayed in contact with air. The ORP rose quickly, mainly because dissolved hydrogen escaped from the water over time, reducing the water’s reducing power. Second, the DPPH assay provided a practical estimate of antioxidant strength. Results indicated only weak antioxidant activity. Specifically, 19 liters of T3 water showed an antioxidant capacity equivalent to 46.2 mg of ascorbic acid for scavenging 1 mmol of DPPH. Overall, ORP suggested promising reducing conditions at first, but the DPPH test showed the real antioxidant effect was limited and decreased further with air exposure.