A procedure to detect 6 basic STSs and 11 extended STSs in the AZF region using multiplex PCR

  • Thi Lan Anh Luong, Thu Lan Hoang, Ngoc Dung Nguyen
  • Thi Lan Anh Luong*, Thu Lan Hoang2, Minh Ngoc Nguyen
Từ khóa: AZF, azoospermia, male infertility, microdeletion, multiplex PCR, oligospermia.

Tóm tắt

Microdeletions of Y chromosomes frequently occur in 3 subregions of the AZF, namely, AZFa, AZFb, and AZFc, with 6 basic STS marker sequences, which are sY84, sY86 (AZFa), sY127, sY134 (AZFb), and sY254, sY255 (AZFc). According to EAA/EMNQ guidelines, 11 additional AZFabc marker sequences should be used to determine the extent of the microdeletion in the AZF region of infertile men, which is known as 11 extended STSs. By applying mPCR, the authors develop an optimal detection procedure for the 6 basic STS and 11 extended STS using 3 multiplex PCR reactions. The first multiplex PCR reaction includes 6 basic STS plus the 2 control sequences sex-determining region Y (SRY)...

Tác giả

Thi Lan Anh Luong, Thu Lan Hoang, Ngoc Dung Nguyen

Hanoi Medical University

Thi Lan Anh Luong*, Thu Lan Hoang2, Minh Ngoc Nguyen

Hanoi Medical University Hospital

điểm /   đánh giá
Phát hành ngày
2021-09-21