Determination on the African swine fever virus (ASFV) inhibitory potential of some herbal ingredients

Abstract

The study was carried out to evaluate the inhibitory ability of 9 herbal ingredients including:
glycyrrhizin, berberine, emodin, ellagic acid, methyl gallate, caffeic acid, kaempferol, baicalein
and naringenin to African swine fever virus (ASFV). The test for cytotoxicity on porcine alveolar
macrophages by these herbal ingredients showed that glycyrrhizin, berberine, ellagic acid,
methyl gallate, kaempferol and naringenin did not cause cytotoxic, emodin, caffeic acid and
baicalein caued cytotoxic at a concentration of 200 µM/ml. The results of testing the ability to
inhibit ASFV of the single substance showed that berberine and emodin showed a significant
ability to inhibit ASFV. After 48 hours of incubating 200 µM/ml of berberine with ASFV, the viral
titer decreased from 107.47 to 105.80; and with emodin at a concentration of 150 µM/ml, the viral
titer decreased from 107.47 to 106.47.