Determination of the F18 fimbrial gene rate and antibiotic susceptibility of E. coli caused diarrhea in post weaning piglet in Huong Son district, Ha Tinh province

Abstract

Study on the rate and frequency carrying F18 fimbrial gene and antibiotic susceptibility of E. coli were conducted from the fecal samples of the diarrheal post weaning piglets collecting in Huong Son district, Ha Tinh province. The studied result showed that there were 17/80 samples( 21.27%) carrying F18 fimbrial gene obtained by applying PCR technique. The distribution frequency of colony bearing F18 fimbrial gene was 0.6. The result of applying diffusion technique on petry disk soaking antibiotic on agar showed that the isolated E. coli strains resisted to several antibiotics at the same time. For example, 100% of the isolated E. coli strains resisted to cefotaxinme, colistin, gentamicin, ofloxacin, tetracycline and trimethoprim. However, these E. coli strains were sensitive with kanamycin, streptomycin and cefoxitin with the rate from 20-80%. The result of this research is a scientific base for selecting an antigen strain to produce vaccine as well as to select some antibiotics that should be used in treatment of diarrhea caused by E. coli in Huong Son district.