UL5 gene decoding and sequence analysis of Duck Enteritis Virus isolated in Viet Nam

Abstract

Duck enteritis virus (DEV) is an agent causing the fatal, contagious, acute disease in all age groups of duck. In this study, the entire of helicase UL5 gene of two DEV strains in Viet Nam including a virulent strain (DTVCDKN) and an attenuated vaccine strain (DTVVXKN) from NationalCentre of Veterinary Medicine Control No.1 was cloned, decoded, it consisted of 2568 nucleotidesencoding 855 amino acids. The composition of DEV UL15 gene included 769 adenine (29.95%), 556 cytosine (21.65%), 533 guanine (20.76%) and 710 thymine (27.65%). Comparisonresult between two Vietnamese DEV UL5 genes and the DEV UL5 genes in GenBank indicatedthat the similarity level of the nucleotide and amino acid of these genes was 100%, while there were 9 different sites of nucleotide leading on 4 different sites of amino acid in comparison between two Vietnamese virus strains and the respective virus strains in GenBank. This is the first report of molecular characterization of DEVs in Viet Nam.