Isolation and selection of bacillus strains for the production of bio-products using in aquaculture

Abstract

95 strains of bacilli were isolated from the samples of shrimp ponds, the worm’s muck for shrimp’s food, and the digestive tract of shrimp. As a result, six strains of Bacilluswere selected. All the selected strains contain the active enzymes(protease, cellulase and amylase), which can strongly disintegrate the organic substances  with (D-d ~ 16,5 - 24,5 mm).  The strains BaD, BaPG, BaRT 9 and BaRT 6 were  chosen for the production  of  bio-products  in  aquaculture. These  Bacillus  strains  showed  strong  antagonistic  properties  against  pathogenic  and  harmful bacteria, and high enzyme activities as well.

The  classification of these strains based on their physiological characteristics  and 16S rRNA sequence indicated that strain BaD which has the similarity rate of  100%  to  Bacillus 63subtilis  EBS05 (FJ876834), strain BaPG with the similarity rate of 99% to the  Bacillus subtilisBFE 5314 (GU250453.1), strain BaPG 3 with the similarity rate of 99% to the  Bacillus subtilis GB16  16S  AY11607.1),  strain  BaRT 6 with the similarity rate of 99% to the Bacillus licheniformis SL2  (GQ184581.1);  The strain BaRT 4 with the similarity rate of  89%  to the Bacillus sp.B26 (2008) (EU362172.1).