Anti-Bacterial Activity of Myrtle Leaf and Myrtle Seed (Rhodomyrtus tomentosa) Extracts on Bacterial Strains Causing Acute Hepatopancreas Necrosis Disease (AHPND) in Shrimp

Abstract

    Acute hepatopancreas necrosis disease (AHPND) has been considered as a major constraint for the sustainable development of shrimp culture industry. This study was carried out to examine in vitro anti-bacterial effects of herbal extracts derived from leaves and seeds of myrtle (Rhodomyrtus tomentosa) on bacterial strains caused AHPND, Vibrio parahaemolyticus KC12.02.0, V. parahaemolyticus KC13.14.2, and Vibrio sp. KC13.17.5. Results showed that although both myrtle leaf and seed extracts had anti-bacterial effects against in vitro AHPND-causing Vibrio strains, the myrtle seed extract showed higher antibacterial activities. The inhibition zone diameters of myrtle seed extract reached 17,67 mm for V. parahaemolyticus KC13.14.2; 18,00 mm for V. parahaemolyticus KC12.02.0 and 19,33 mm for Vibrio sp. KC13.17.5. These results suggested myrtle seed extract can potentially be used as a material for development of herbal medicine for AHPND control.