Molecular Characterization of The Porcine Epidemic Diarrhea Viruses Isolated in Quang Tri, Thai Nguyen, and Thai Binh During 2013 – 2014

Abstract

    Porcine Epidemic Diarrhea (PED) is a serious and contagious swine disease caused by a virus of Coronaviridae family. PED occurs throughout the year but more common in winter season and over 90% of cases occur in less than 7 day old piglets. In this study, 148 samples of faeces and intestines collected from PED-suspected pigs in the 3 provinces of Quang Tri, Thai Nguyen and Thai Binh during 2013 - 2014 were diagnosed by the RT-PCR method. The diagnostic results showed that 57/148 (38.51%) samples were positive with the PED virus. The nucleotide (nt) and deduced amino acid (aa) analyses based on the partial spike (S) gene sequencing showed that the present PED virus strains were closely related to the reference PED virus strains collected in Viet Nam previously, sharing 94.8 - 98.6% in nt and 93.5 - 98.8% in aa. The phylogenetic analysis revealed that all the present PED virus strains belonged to the group G1-1.