Identification and Evaluation of Some Characteristics of Actinomyces Isolates as Potential Agents for Controling Anthracnose Disease on Momordica cochinchinensis

Abstract

     The research was carried out in the laboratory of Plant Protection department, Can Tho University. The airms of this research were to (i) identify the actinomyces isolates that were able to control anthracnose disease on Momordica cochinchinensis and (ii) examine their characteristics. Based on the culture, morphological, physiological and biochemical characteristics and sequencing of the16S-rRNA gene, five actinomycetes isolates were identified as five species: Streptomyces avellaneus, Streptomyces pallidus, Streptomyces vinaceus, Streptomyces showdoensis and Streptomyces exfoliatus. Chitinase activity assay tested on chitin medium showed that S. pallidus and S.vinaceus isolates had chitinolytic activity, with the chitin lyses halo radius of 13.6 mm and 12.9 mm, respectively, at 7 days after testing. β-glucanase activity assay tested on β-glucan medium indicated that S. avellaneus had the β- glucanolytic activity, with the β-glucan lyses halo radius of 6.0 mm at 13 days after testing.