in vitro Propagation of Coelogyne mooreana Sander Ex Rolfe

Abstract

      In this study, in vitro mass propagation of Coelogyne mooreana Sander ex Rolfe, an endemic orchid (of Vietnam) with its beautiful large, white flowers and fragrance having horticultural potential was accomplished through protocorm formation from shoot tips and subsequent plant regeneration. In the first procedure, shoot tips from in vitro germinated seedlings were cultured on Murashige and Skoog medium (MS) supplemented with a range of 6 - benzylaminopurine (BA) in combination with naphthalene acetic acid (NAA) to determine the best medium for the protocorm formation. The results showed that MS medium supplemented with 1.0 mg/l BA and 0.5 mg/l NAA was the most suitable for protocorm formation (6.62 protocorms và formation rate of 86.63%). For subculture, maximum growth of shoots (14 shoots, 2.72 cm height and 3 leaves/shoot) was obtained on MS medium supplemented with 0.3 mg/l NAA and 2.0 mg/l BA. The highest frequency of rooting (100%) was observed on ½MS medium supplemented with 20 g/l sucrose, 8 g/l agar, 1 g/l activated charcoal (AC), 10% coconut water (CW) and 0.5 mg/l NAA. On this medium, the seedlings reached an average length of 1.66 cm with 3.8 roots per seedling. A successful protocol for micropropagation by shoot tips will contribute to the development of a suitable management program of C. mooreana Sander ex Rolfe.