STUDY ON ADVENTITIOUS ROOT FORMATION OF TONGKAT ALI TREE (Eurycoma longifolia Jack.) BY IN VITRO CULTURE

Abstract

Tongkat ali tree (Eurycoma longifolia Jack.) is a medicinal plant of Simaroubaceae family. It has been marketed for the supposed benefits of general and sexual health improvement. In this study, the medium for adventitious root induction of Eurycoma longifolia was investigated. The results indicated that MS medium supplemented with combinations of BA 0,1 mg/L and NAA 1,5 mg/L was optimum for adventitious root induction in cotyledon explants, while MS medium supplemented with glucose 40 g/L was optimum for adventitious root induction in callus. This in vitro strategy has the potential to hasten the development of a genetically stable system for the enhanced production of secondary metabolite in culture of Eurycoma longifolia plants.

Keywords: Adventitious root, BA, callus, Eurycoma longifolia, NAA