INVESTIGATION OF FACTORS AFFECTING THE ABILITY OF PECTINASE BIOSYNTHESIS BY Aspergillus niger USING ORANGE PEELS AS SUBSTRATE
Abstract
Pectinase from microorganisms accounts for 25% of the global food enzyme sales. Almost all the commercial preparations of pectinase are produced from mold. Aspergillus niger is most commonly used for the production of pectinase. The study considered factors affecting the biosynthesis of pectinase by Aspergillus niger on liquid medium supplemented with inducer substrate source from orange peel. The results of the experiments were evaluated by pectinase enzyme activity. The results showed that the pectinase enzyme had the highest activity of 194 UI/mL when Aspergillus niger was cultured on liquid medium with 80 g/L orange peel (0.72 g/L pectin content), orange peel to glucose ratio of 4:2, suitable nitrogen source peptone 4% (w/v), seed rate 2% (v/v), density 107 spores/mL, pH 5.0 and culture period of 72 hours. This culture medium can produce pectinase enzyme by Aspergillus niger and it can be applied in the fruit juice industry.
Keywords: Mold, Aspergillus niger, pectin, pectinase, orange peel.
