THE EFFECTS OF LIGHT EMITTING DIODE ON GROWTH, ANTIOXIDANT ACTIVITY AND TOTAL PHENOLIC CONTENT OF Ehretia asperula Zoll. & Mor. CULTURED IN VITRO

Abstract

Ehretia asperula Zoll. & Mor. contains compounds such as flavonoid, saponin triterpenoid, quinon. In this paper, the effects of kinetin on the shoot formation derived from ex vitro shoot and effect LED (Light emitting diode) on the growth plant, antioxidant activity, and total phenolic content were studied. Ex vitro-derived nodes were cultured on MS medium supplemented with KIN at a concentration of 3.0 mg/L, the shoot formation ratio (100%) and shoots per explant (1.57 shoots/explant) were the highest one. The Ehretia asperula shoots of 2,0 cm high were cultured in MS medium without plant growth regulators, and incubated under six different lighting conditions: monochromatic red LED (R), monochromatic blue LED (B), red and blue LED mixtures (50R:50B, 70R:30B), growth light and fluorescent lamp light (control experiment). After 4 weeks of culture, in vitro plants cultured  under the growth light condition reached the highest level of fresh weight, plant height and leaf number (1.57 g, 4.46 cm and 9.11 leaves, respectively). In addition, the explants cultured under the growth light conditions showed antioxidant capacity (IC₅₀ = 0.1166 mg/mL) and total phenolic content (81,06 mg GAE/g extract) higher than those cultured under other light conditions. Besides, there are many secondary compounds such as carbohydrate, protein - amino acid, lipid in extract of E. asperula plant.

Keywords: Antidioxidant activity, phenolic content, in vitro, growth, Ehretia asperula.