NUCLEOTIDE SEQUENCE OF CYTOCHROME B OF SAOLA (PSEUDORYX NGHETINHENSIS)

Abstract

We investigated the systematic position of Saola (Pseudoryx nghetinhensis) in A Luoi (Thua Thien Hue) using the cytochrome b region. DNA was cxtracted from tissue of born and hom. The nucleotide sequence of cytochromc b was determined with 333 bp and used to analysis of phylogeny of Saola using neighbor joining method, together with 8 mammal species including Bas javanicus, B. sauveli, B. gaurus, Capricornis sumatraensis and Bubalus amee (Bovidae), Axis porcinus and eervus eldi (Cervidae), Maschus berezavski (Mischidae), and Saola (Pseudoryx nghetinhensis) in Vu Quang (Ha Tinh). The analysis indicated that Saola in Vu Quang and A Luoi formed a cluster with bootstrap 100%. The results also indicated that very low level of the sequence divergencc was found in cytochrome b between Saola in Vu Quang and A Luoi (3.8%). The bootstrap supports monophyletic clade of Bos group including B. juvunlcus, B. suuveli and B. gaurus in Bovidae.