NGHIÊN CỨU ỨNG DỤNG CHYLOMICRON TÀN DƯ TRONG HỆ VẬN CHUYỂN THUỐC TỚI GAN

Tóm tắt

Chylomicrons (CM) are the largest lipid particles in the blood which consist largely of triglycerides.
Chylomicron remnants (CMr) are formed when peripheral tissue lipids are removed from chylomicrons
and bind to receptors on the surface of hepatocyte. Due to its properties, chylomicron remnants can
serve as a great vehicle for drug delivery. This paper focuses on studying a three-component CMr -
Toc - siRNA system, thereby improving the effectiveness of fatty liver disease treatments and reducing
the effects unwanted side. The CMr – Toc – siRNA complexes were synthesized by lipophilic binding of
the tocopherol residue of Toc-siRNA and CMr. The system was, then, tested for cytotoxicity by
incubation with the Live/Dead assay kit and testing in mice to determine total RNA. According to
research, chylomicron remnants have an average diameter of 140-143 nm determined by dynamic light
scattering (DLS) measurement and contain apoB48 and apoE. With a cell viability of approximately
92.2%, the chylomicron remnant system carrying siRNA is highly biologically safe. Results from qRTPCR showed that CMr-Toc-siRNA was effective at delivering siRNA to liver cells via chylomicronmediated siRNA delivery, since endogenous apoB mRNA levels decreased by more than 30%.