NGHIÊN CỨU PHƯƠNG PHÁP RT-PCR SỬ DỤNG CHẤT NHUỘM HUỲNH QUANG SYBR GREEN ĐỂ ĐỊNH LƯỢNG HPV-16

Tóm tắt

HPV16 is the most common high-risk human papillomavirus genotype causing over haft of all cervical
cancer cases in women worldwide. The quantification of HPV-16 plays a key role in the diagnosis and
treatment of cervical cancer. In this study, we investigated the specificity of primers and optimized the
conditions of RT-PCR reaction for detection of HPV-16: temperature annealing 560C; primers
concentration: 0,25 M; thermal cycles: 35 cycles; SYBR Green concentration: 0,25x. Based on the
optimal conditions of RT-PCR reaction established applied to determine HPV-16 in 23 real specimens.
The results are similar to those of HPV-16 analysis on IVD standard kits.