PHÂN TÍCH DƯ LƯỢNG MỘT SỐ KHÁNG SINH TRONG NƯỚC THẢI BỆNH VIỆN BẰNG PHƯƠNG PHÁP SẮC KÝ LỎNG SIÊU HIỆU NĂNG CAO GHÉP NỐI KHỐI PHỔ PHÂN GIẢI CAO (UPLC- ORBITRAP MS)

Tóm tắt

This work has introduced method UPLC- Orbitrap MS to determine six antibiotic compounds
(Ciprofloxacin, Cephalexin, Moxifloxacin, Clarithromycin, Levofloxacin) in wastewater collected from
several hospitals in Vietnam. The chromatographic procedure used Zorbax-eclipse XDB C18 100 x 4,6
mm, 3,5 µm (Agilent) and 0.1% FA in deionized water and MeOH as mobile phases. Selected ion
monitoring (SIM) mode was used for qualification and qualitication of target analytes. The parameters
such as: temperature column, mobile phases, gradient, flowrate were optimized. The investigated
compounds were cleanup from match-matrix based on SPE extraction using HLB column(supelco). The
spiked experiments were carried out for method evaluation through several parameters, such as:
repeatability, recovery (85,2 ± 10,1% - 107,6 ± 3,5%), linearity, MDL 0,03 ng/L (Cephalexin,
Amoxicillin) – 0,07 ng/L (Clarithromycin, Ciprofloxacin, Levofloxacin) và LOQ nằm trong khoảng từ
0,10 ng/L (Cephalexin) – 0,23 ng/L (Clarithromycin). Applying this method, 30 real samples collected
from five hospitals in our country were analyzed. It can be clearly seen that their systems of treatment
wastewater have been operated effectively.