NGHIÊN CỨU XÁC ĐỊNH CHLOROPHYLL A BẰNG PHƯƠNG PHÁP SẮC KÝ LỎNG HIỆU NĂNG CAO

Tóm tắt

A simple High-Performance Liquid Chromatographic (HPLC) method was developed and validated for
the determination of Chlorophyll a in phytoplankton and water. The separation was carried out by a
Nova-Pak C18 column (150 mm × 3.9 mm; 4 µm) with mobile phase consisted of acetone: metanol
(70:30 v/v). The flow rate was 0.6 mL/min and DAD detection at 430 nm. The retention time of
Chlorophyll a was found at 4.8 min. The calibration curves were linear with the regression coefficient
(R2
) of 0.9999 within the range from 0.005 to 5ppm. The detection and quantification limit were 0.002
and 0.005 ppm, respectively. This method was used to found sensitive, specific, and capable of
estimation of Chlorophyll a in phytoplanktons and water. This method has been applied successfully,
sensitively and specifically in the determination of Chlorophyll a in phytoplankton and water.