THẨM ĐỊNH PHƯƠNG PHÁP VÀ ỨNG DỤNG PHÂN TÍCH ĐỒNG THỜI CÁC ĐỘC TỐ AFLATOXIN TRONG MẪU ĐẬU PHỘNG BẰNG PHƯƠNG PHÁP SẮC KÝ LỎNG GHÉP KHỐI PHỔ

Tóm tắt

Aflatoxin is the high toxicity group of mycotoxins that can lead to fatal if acute poisoning or liver
cancer if accumulating at low levels in the body. In this study, an ultra-performance liquid
chromatography-tandem trip-quadruple mass spectrometry (UPLC-MS/MS) method has been validated
for simultaneous determination of aflatoxins group B and G in produced peanut samples. Under
optimal experimental conditions, the method detection limit (MDL) for aflatoxin B1 (AFB1) and
aflatoxin G1 (AFG1) are 0,1 µg/kg. MDL for aflatoxin B2 (AFB2) and aflatoxin G2 (AFG2) are 0,03
µg/kg. The correlation coefficient (R2
) of all calibration lines are 0.999. The average method recovery
(%) at three different levels and for all four aflatoxins range from 90,7 to 98%. At different
concentrations of aflatoxins, the relative standard deviations (RSD%) of repeatability is lower than 1,1
%, and the intermediate precision is lower 3.8 %. Comparison with the requirements of the Viet Nam
Ministry of Health, the validated method is very sensitive and accurate to determine the aflatoxin in
peanut product sample, and can be used for quality control and food safety.