GENOME COMPARATIVE OF EDWARDSIELLA REVEALS POTENTIAL DEVELOPMENT OF SUSTAINABLE PROPHYLAXES

Tóm tắt

Aquatic diseases caused by the massive wealth of pathogenic bacteria pose major challenges to the
development of a sustainable bio-control method, such as antimicrobial measures and vaccine strategies.
Recent advances in genome sequencing technology have revolutionized the field of pathogenic pan-genomics
and have also influenced disease management in aqua farms. In this study, Edwardsiella strains were
differentially classified into four species by a phylogenomics construction based on the pan-genome analysis.
Edwardsiella species were correctly classified by pan-genome analysis (core gene, dispensable gene,
singleton gene) of 15 complete genomes. Based on the presence of the gene repertoires, gene encoding
extracellular protein, outer membrane protein, adhesion ability and antigenic sites, 9 genes (E. ictaluri), 13
genes (E. anguilarium), 9 genes (E. piscicida), 12 genes (E. tadar), and 14 genes (all species) screened from
core-gene of 2686, 2673, 2877, 2920, and 1957 gene, respectively have potential in developing reverse
vaccinology strategy to the prevention of Edwarsiellosis. The in-silico analysis will also help to optimize the
time and improve the cross-serotype reaction of vaccines in farmed fish. The RV research implementing pangenome analysis will be a strategy that is applicable to pathogens in both aquatic and terrestrial animals.