Shoots formation from gynosinesis cucumis sativus l.

Abstract

    Haploid plants achieve through androgenesis or gynogenesis. In gynogenesis method, the ovary or ovule areused as explants induct haploid plants. Female flower one day before flowering of Cucumis sativus L. are collected.Cold pretreatment of ovaries at 4°C up to 24 hours and culture under dark conditions. Significantly enhanced callusinduction response is compared with cultures under 4-week cultured on CBM medium supplemented with variousconcentration of TDZ 0.01-0.04 mg/L. After 4 weeks, ovaries are transferred to medium with kinetin 0.05 - 0.20mg/L. Then, ovaries were transferred to medium supplemented with BA: IAA 3:1. Finally, green ovaries weretransferred to BA 1.5 mg/L and GA3 1.5 mg/L. The results showed that ovary induction has best affected on CBMwith TDZ 0.03 mg/L with 11 callus/sample. Ovaries developed on kinetin 0.1 mg/L with 7.4 callus/sample. Ovariesbecome green and had leaves and roots formation on BA: IAA (3 mg/L: 1 mg/L). 11 plantlets were harvested fromovary culture after 12-week culture on CBM supplemented with BA 1.5 mg/L and GA3 1.5 mg/L.