Study on Potato In - vitro Regeneration System for Gene Transfering and Protoplast Fusion

Abstract

The investigation was conducted to develop a protocol for callus induction and plant regeneration of potato (Solanum tuberosum L). The internodal and leaf explants of  the variety Diamant (2n = 4x) and 3 diploid clones A15, H1959/195, H1929/34 (2n = 2x) were cultured for callus induction and plant regeneration on MS medium supplemented with different types of sugars (sucrose, glucose, manitol) and various concentrations  of a-NAA (0.1 - 0.5 ppm), 2.4 -D (0.1 - 0.25 ppm); BA (1 - 3 ppm) and kinetin (0.5 - 3 ppm). Of all cultivar and clones, the callusing response of both types of explants was rather high with the rate from 70.0% to 100% for Diamant and 21.4 - 97.5% for diploid clones. Highest shoot formation (78.6%) was obtained from calli derived from internodal explants of Diamant cultured on MS medium contaning 15 g saccharose + 30 g manitol  + 5 g glucose + 2 mg/l BA. This medium also gave the highest rate of shoot regeneration from internode induced calli of diploid clone H1959/195 (52.6%). The internodes excised from different position showed different results on suvival rate and shoot regeneration. The highest suvival rate was obtained with the basal internodes. The morphology of regenerated plants appeared to be similar to that of the control plants when grown in the field