EFFECT OF FREE FATTY ACID ON EXPRESION OF LIGHT AND HVEM mRNA LEVELS IN CULTURED SKELETAL MUSCLE CELLS

DOI: 10.18173/2354-1059.2020-0054

  • Le Ngoc Hoan
  • Chu Dinh Toi
  • Ho Thi Hong Van
Từ khóa: Free fatty acid, skeletal muscle cells, light/HVEM.

Tóm tắt

Light, tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted peptide of the TNF superfamily. It binds to the herpesvirus entry mediator (HVEM). Light\HVEM signaling is well reported as an important factor linking inflammation and obesity. Here, in the current study, we manipulated skeletal muscle cells incubated in media containing free fatty acid (FFA) to mimic an obesity-related inflammatory environment. Our result revealed that FFA strongly induced expression of mRNA levels of light and interleukin 6 (IL6) - an inflammatory cytokine in the skeletal muscle cells. Surprisingly, expression of HVEM mRNA levels was not significantly different between the FFA-treated skeletal muscle cells and the control cells. These data suggest that FFA increases light expression in skeletal muscle cells that in turn, can bind to HVEM as an autocrine effect inducing inflammatory responses in skeletal muscle cells.

điểm /   đánh giá
Phát hành ngày
2021-05-10
Chuyên mục
BAI BÁO