Evaluation the effect of several anticancer drugs on Vietnamese breast cancer cells

Tóm tắt

In Viet Nam, data from Conference of Cancer organized by the Ministry of Health has shown
that breast cancer is the most popular cancer in women. Current mainly treatments are surgery,
chemotherapy, and radiotherapy. However, the rate of recurrence after five years was very high.
One of the causes of high relapse is cancer cells develop multidrug-resistant (MDR) thus reduced
the efficiency of treatments. In this research, MTT assay was used for measured cell viability of Vietnamese
breast cancer cells (VNBRCA cells) and positive control MCF-7 cell lines after treatment with
several anticancer drugs as Doxorubicin (DOX), Tamoxifen (TAM), Mitomycin C (MMC) in 48h. After
that, cancer cells were treated at haft maximal inhibitory concentration (IC50) of anticancer drug
and observed cell morphology, apoptosis of cellular nuclear by AO/PI staining. IC50 value of VNBRCA
cells with DOX, TAM, MMC were 0.641 0.07 M, 4.639 0.933 M and 1.338 0.176 M,
respectively, which higher than IC50 of MCF-7 with DOX, TAM, MMC was 0.1680.037 M, 7.085 0.87 M and 0.379 0.159 M, respectively. The response of VNBRCA cells with several anticancer
drugs as DOX, TAM, and MMC was lower than the response of MCF-7, therefore, it showed that the
specific features of VNBRCA cells; from which develop specific treatments for Vietnamese breast
cancer patients.