PRIMARY INVESTIGATION OF LISTERIA MONOCYTOGENES INFECTION IN FOODS IN HANOI MARKET USING POLYMERASE CHAIN REACTION

Abstract

Using PCR techniques developed with LM-F76, LM-R543 primers, the investigation oftheinfection of L. monocytagenes was done with 165 food samples including 116 dairy- and 49meat-based foods. The PCR analysis showed no contamination with L. monocylogenes wasdetected in dairy samples, in contrary, 11149 meat samples was positive with L. monocylogenes. This result was verified by identification of the strains isolated from positive sample on Oxfordagar with 16S rDNA technique. The result confirmed that all strains isolated from PCR positivesamples were identified as L. monocytagenes. This primary analysis gave a first warning to thecontamination risk of L. manocylogenes in food in Hanoi market. It demands a widely investigation for the dead bacterium infection risk in the marketed foods.