SERIAL RELEASE TESTING FOR FAST, SPECIFIC KIT THAT DETECTING ANTIBODIES AGAFNST Salmonella enteritidls IN CHICKEN SERA

Abstract

It  is  necessary  tP  test  spme  parameters  of  kit  that  detecting  fast,  specifically  antibodies against  S.  enteritidls  in  chicken  sera  before  commercialization  to  avoid  excessive  variation between  serials. Two chicken  sera  samples containing  antibodies  against  5". enteritidls (positive sample) and  2 chicken  sara negative  samples  were  used  for  our experiment.  This  kit had  100% of  sensitivity  and  93.3% of  specificity.  The  cut-off  line  of this  kit  was  0.106.  The  SD and CV was calculated  base on OD mean  value. The obtained  results  shown  that the calculated  SD was below  10%). The theoretical  cut-off  to the kit cut-off  had been to lower than  1 with the nagative
samples, while this  ratio  gave  from  positive  samples  alway  higher  than  4. Therefore,  all  of the tested  negative  sera  samples  and positive  sera  samples were released  the results  satisfactory.  At the same time, the kit serials were also tested  for repeatability  and  reproducibility  of tested  sera samples.  The  obtained  results  shown  that  this  kit  was  released  very  stable  results  in  both  of negative  and  positive  sera  sample. It means that the  same  results were  obtained  for  one sample when  it was applied on a microplate but also on different  serials.