DETERMINING OPTIMAL CONDITIONS TO FORMING GALACTOOLIGOSACCHARIDES (GOS) BY TRANSGAIACTOSYL REACTION USING P-GALACTOSIDASE PRODUCED BY ASPERGILLUS ORYZAE 3
Abstract
in the present paper, enzyme P-galactosidase from A.oryzae 3 was used for the production GOS from lactose. The optimal conditions to forming GOS by transgalactosyl reaction using this enzyme were determined, such as 8 hours at 55°C with 60% of lactose concentration (w/v),7 U/ml of enzyme concentration and pH 5.5. The GOS production process was established and the total GOS yield (%o) determined by HPLC were more than 50%.