Nghiên cứu chế tạo và khảo sát hiệu ứng kích kháng bệnh gan thận mủ trên cá tra (Pangasianodon hypophthalmus) của Oligochitosan và OligoB-Glucal

Abstract

OligoP-glucan and oligochitosan were prepared by gamma Co-60 irradiation of p- glucan/H202 and chitosan/H202 solution. The efficiency of the degradation process was determined by gel permeation chromatography (GPC) method. Results showed that the Mw decreased with increasing concentration of H2O2 and doses. For oligoP-glucan, Mw reduced from 56.7 kDa to 7.1 kDa when p-glucan 10 %/H202 1 % solution was iưadiated at 14 kGy. For oligochitosan, Mw reduced from 45.5 kDa to 5.0 kDa when chitosan 4 %/H202 0.5 % solution was iưađiated at 21 kGy. Tra catfish (Pangasianodon hypophthalmus) was fed with oligop- glucan and oligochitosan in various concentrations of 0, 50, 100, and 200 mg/kg feed for 45 days and then was challenged with EdwardsieUa ictaluri bacteria to investigate immune stimulation effect against white patches in the internal organs disease. The results indicated that oligoP- glucan and oligochitosan exhibited good immune stilmulation effect with optimum concentration of 100 mg/kg feed.