Genetic variation and population structure in Pinus krempfii lecomte - endemic species in Tay Nguyen, Vietnam by ISSR markers

Abstract

Highland IS one of the areas where is abundant with coniferous species of Vietnam. Most of these species are economic and of scientific value. Many coniferous species are in danger of being threatened with extinction, among them is Pinus krempfii species - endemic in Vietnam. Therefore, surveys of species status and the conducting some basic researchs are the scientific basis for the conservation, rational use and sustainable development. Results of the molecular analysis based on ISSR markers were used to study genetic diversity of Piniis krempfii populations collected in Giang Ly, Cong Troi, Hon Giao and Chu Yang Sin. There were 18/26 primers revealed polymorphic. Among 137 fragments were amplified, of which 98 were polymorphic (accounting for 71.53 %). The genetic diversity is the highest in Chu Yang Sin populations (h = 0.117; I = 0.239; PPB = 62.04; Ne = 1,.226 and He = 0.151) and lowest in Giang Ly population Ọi = 0.070; I = 0,086; PPB = 13.87; Ne = l.lll and He - 0.060). The degree of genetic differences identified among populations is low (total variation is 20.28 %) and higher within populations (79.82 %). A phylogenetic tree based on UPGMA analysis grouped the 70 genotypes into two main clusters with genetic similarity within clusters ranged from about 65.7 (Pk45 and Pk8) to 100 % (Pkl2 and Pkl3; Pk47 and Pk48). These results suggest that Pinus krempfii populations should be paid attention to the conservation of species.