Extraction and purification of collagen from the skins of basa fish (Pangasius hypophthalmus)

  • Lê Thị Thu Hương
  • Nguyễn Hoàng Dũng
  • Phan Đình Tuấn

Abstract

Acid-solubilized collagen (ASC) was extracted from basa fish skin (Pangasius hypophthalmus) by acetic acid and purified by dialysis, lyophilization and supercritical carbon dioxide (SC-CO2) extraction techniques. After 24 h under stirring condition, more than 93 % of salt was removed by dialysis. The efficiency of lipid extraction process reached 91-6 % at the following conditions: pressure of 200 bar, CO2 flow rale of 10 g/min, temperature of 38 °c, concentration of ethanol as co-solvent of 10 % (v/v) and the retention time of 60 minutes. The obtained collagen has low impurities of lipid (0.33 %), and ashes (0.29 %). Moreover, extracted collagen has no smell, no color, high molecular weight, and is appropriate for cosmetic purposes, pharmaceutical and food applications.

điểm /   đánh giá
Published
2017-08-30
Section
Articles